Temperature response of isoprene emission in vivo reflects a combined effect of substrate limitations and isoprene synthase activity: a kinetic analysis.
Identifieur interne : 003096 ( Main/Exploration ); précédent : 003095; suivant : 003097Temperature response of isoprene emission in vivo reflects a combined effect of substrate limitations and isoprene synthase activity: a kinetic analysis.
Auteurs : Bahtijor Rasulov [Estonie] ; Katja Hüve ; Irina Bichele ; Agu Laisk ; Ulo NiinemetsSource :
- Plant physiology [ 1532-2548 ] ; 2010.
Descripteurs français
- KwdFr :
- MESH :
- enzymologie : Populus.
- métabolisme : Alkyl et aryl transferases, Butadiènes, Hémiterpènes, Pentanes, Populus.
- Cinétique, Photosynthèse, Régulation de l'expression des gènes végétaux, Température.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Alkyl and Aryl Transferases, Butadienes, Hemiterpenes, Pentanes.
- enzymology : Populus.
- metabolism : Populus.
- Gene Expression Regulation, Plant, Kinetics, Photosynthesis, Temperature.
Abstract
The responses of isoprene emission rate to temperature are characterized by complex time-dependent behaviors that are currently not entirely understood. To gain insight into the temperature dependencies of isoprene emission, we studied steady-state and transient responses of isoprene emission from hybrid aspen (Populus tremula × Populus tremuloides) leaves using a fast-response gas-exchange system coupled to a proton-transfer reaction mass spectrometer. A method based on postillumination isoprene release after rapid temperature transients was developed to determine the rate constant of isoprene synthase (IspS), the pool size of its substrate dimethylallyldiphosphate (DMADP), and to separate the component processes of the temperature dependence of isoprene emission. Temperature transients indicated that over the temperature range 25°C to 45°C, IspS was thermally stable and operated in the linear range of its substrate DMADP concentration. The in vivo rate constant of IspS obeyed the Arrhenius law, with an activation energy of 42.8 kJ mol(-1). In contrast, steady-state isoprene emission had a significantly lower temperature optimum than IspS and higher activation energy. The reversible temperature-dependent decrease in the rate of isoprene emission between 35°C and 44°C was caused by decreases in DMADP concentration, possibly reflecting reduced pools of energetic metabolites generated in photosynthesis, particularly of ATP. Strong control of isoprene temperature responses by the DMADP pool implies that transient temperature responses under fluctuating conditions in the field are driven by initial DMADP pool size as well as temperature-dependent modifications in DMADP pool size during temperature transients. These results have important implications for the development of process-based models of isoprene emission.
DOI: 10.1104/pp.110.162081
PubMed: 20837700
PubMed Central: PMC2971629
Affiliations:
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substrate limitations and isoprene synthase activity: a kinetic analysis.</title><author><name sortKey="Rasulov, Bahtijor" sort="Rasulov, Bahtijor" uniqKey="Rasulov B" first="Bahtijor" last="Rasulov">Bahtijor Rasulov</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia.</nlm:affiliation><country xml:lang="fr">Estonie</country><wicri:regionArea>Institute of Molecular and Cell Biology, University of Tartu, Tartu</wicri:regionArea><wicri:noRegion>Tartu</wicri:noRegion></affiliation></author><author><name sortKey="Huve, Katja" sort="Huve, Katja" uniqKey="Huve K" first="Katja" last="Hüve">Katja Hüve</name></author><author><name sortKey="Bichele, Irina" sort="Bichele, Irina" uniqKey="Bichele I" first="Irina" last="Bichele">Irina Bichele</name></author><author><name sortKey="Laisk, Agu" sort="Laisk, Agu" uniqKey="Laisk A" first="Agu" last="Laisk">Agu Laisk</name></author><author><name sortKey="Niinemets, Ulo" sort="Niinemets, Ulo" uniqKey="Niinemets U" first="Ulo" last="Niinemets">Ulo Niinemets</name></author></analytic><series><title level="j">Plant physiology</title><idno type="eISSN">1532-2548</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alkyl and Aryl Transferases (metabolism)</term><term>Butadienes (metabolism)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Hemiterpenes (metabolism)</term><term>Kinetics (MeSH)</term><term>Pentanes (metabolism)</term><term>Photosynthesis (MeSH)</term><term>Populus (enzymology)</term><term>Populus (metabolism)</term><term>Temperature (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alkyl et aryl transferases (métabolisme)</term><term>Butadiènes (métabolisme)</term><term>Cinétique (MeSH)</term><term>Hémiterpènes (métabolisme)</term><term>Pentanes (métabolisme)</term><term>Photosynthèse (MeSH)</term><term>Populus (enzymologie)</term><term>Populus (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Température (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Alkyl and Aryl Transferases</term><term>Butadienes</term><term>Hemiterpenes</term><term>Pentanes</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Alkyl et aryl transferases</term><term>Butadiènes</term><term>Hémiterpènes</term><term>Pentanes</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Kinetics</term><term>Photosynthesis</term><term>Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cinétique</term><term>Photosynthèse</term><term>Régulation de l'expression des gènes végétaux</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The responses of isoprene emission rate to temperature are characterized by complex time-dependent behaviors that are currently not entirely understood. To gain insight into the temperature dependencies of isoprene emission, we studied steady-state and transient responses of isoprene emission from hybrid aspen (Populus tremula × Populus tremuloides) leaves using a fast-response gas-exchange system coupled to a proton-transfer reaction mass spectrometer. A method based on postillumination isoprene release after rapid temperature transients was developed to determine the rate constant of isoprene synthase (IspS), the pool size of its substrate dimethylallyldiphosphate (DMADP), and to separate the component processes of the temperature dependence of isoprene emission. Temperature transients indicated that over the temperature range 25°C to 45°C, IspS was thermally stable and operated in the linear range of its substrate DMADP concentration. The in vivo rate constant of IspS obeyed the Arrhenius law, with an activation energy of 42.8 kJ mol(-1). In contrast, steady-state isoprene emission had a significantly lower temperature optimum than IspS and higher activation energy. The reversible temperature-dependent decrease in the rate of isoprene emission between 35°C and 44°C was caused by decreases in DMADP concentration, possibly reflecting reduced pools of energetic metabolites generated in photosynthesis, particularly of ATP. Strong control of isoprene temperature responses by the DMADP pool implies that transient temperature responses under fluctuating conditions in the field are driven by initial DMADP pool size as well as temperature-dependent modifications in DMADP pool size during temperature transients. These results have important implications for the development of process-based models of isoprene emission.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20837700</PMID><DateCompleted><Year>2011</Year><Month>02</Month><Day>10</Day></DateCompleted><DateRevised><Year>2019</Year><Month>01</Month><Day>08</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1532-2548</ISSN><JournalIssue CitedMedium="Internet"><Volume>154</Volume><Issue>3</Issue><PubDate><Year>2010</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Temperature response of isoprene emission in vivo reflects a combined effect of substrate limitations and isoprene synthase activity: a kinetic analysis.</ArticleTitle><Pagination><MedlinePgn>1558-70</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1104/pp.110.162081</ELocationID><Abstract><AbstractText>The responses of isoprene emission rate to temperature are characterized by complex time-dependent behaviors that are currently not entirely understood. To gain insight into the temperature dependencies of isoprene emission, we studied steady-state and transient responses of isoprene emission from hybrid aspen (Populus tremula × Populus tremuloides) leaves using a fast-response gas-exchange system coupled to a proton-transfer reaction mass spectrometer. A method based on postillumination isoprene release after rapid temperature transients was developed to determine the rate constant of isoprene synthase (IspS), the pool size of its substrate dimethylallyldiphosphate (DMADP), and to separate the component processes of the temperature dependence of isoprene emission. Temperature transients indicated that over the temperature range 25°C to 45°C, IspS was thermally stable and operated in the linear range of its substrate DMADP concentration. The in vivo rate constant of IspS obeyed the Arrhenius law, with an activation energy of 42.8 kJ mol(-1). In contrast, steady-state isoprene emission had a significantly lower temperature optimum than IspS and higher activation energy. The reversible temperature-dependent decrease in the rate of isoprene emission between 35°C and 44°C was caused by decreases in DMADP concentration, possibly reflecting reduced pools of energetic metabolites generated in photosynthesis, particularly of ATP. Strong control of isoprene temperature responses by the DMADP pool implies that transient temperature responses under fluctuating conditions in the field are driven by initial DMADP pool size as well as temperature-dependent modifications in DMADP pool size during temperature transients. These results have important implications for the development of process-based models of isoprene emission.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rasulov</LastName><ForeName>Bahtijor</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hüve</LastName><ForeName>Katja</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Bichele</LastName><ForeName>Irina</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Laisk</LastName><ForeName>Agu</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Niinemets</LastName><ForeName>Ulo</ForeName><Initials>U</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. 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sortKey="Niinemets, Ulo" sort="Niinemets, Ulo" uniqKey="Niinemets U" first="Ulo" last="Niinemets">Ulo Niinemets</name></noCountry><country name="Estonie"><noRegion><name sortKey="Rasulov, Bahtijor" sort="Rasulov, Bahtijor" uniqKey="Rasulov B" first="Bahtijor" last="Rasulov">Bahtijor Rasulov</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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